Table 3.

Summary table of cognitive tests performed in mice immunized with MOG₃₅₋₅₅ (QuilA-EAE) and their controls (CTL) during the chronic phase of the disease (D30).

Object recognition test % of recognition		Y-maze % of alternation		Elevated plus maze, Time in open arms (s)		Marble burying test, Number of marbles buried		Open field test, Time spent in central area (s)
Cohort 1 (n = 7)		Cohort 2 (n = 12)		Cohort 2 (n = 12)		Cohort 2 (n = 12)		Cohort 3 (n = 12)
CTL 67.43 ± 4.87 EAE 59.86 ± 0.94	NS	CTL 56.41 ± 2.50 EAE 55.93 ± 2.35	NS	CTL 17.53 ± 3.60 EAE 23.83 ± 4.58	NS	CTL 9.08 ± 1.52 EAE 8.83 ± 1.20	NS	CTL 94.38 ± 14.8 EAE 78.68 ± 9.02	NS
Cohort 2 (n = 8–12)		Cohort 3 (n = 12)		Cohort 3 (n = 12)		Cohort 3 (n = 12)		Cohort 4 (n = 12)
CTL 67.64 ± 2.18 EAE 62.97 ± 2.23	NS	CTL 56.50 ± 2.59 EAE 55.90 ± 2.47	NS	CTL 25.29 ± 7.11 EAE 17.52 ± 5.08	NS	CTL 6.58 ± 1.19 EAE 5.83 ± 0.83	NS	CTL 78.92 ± 11.4 EAE 74.38 ± 10.46	NS

Episodic memory was assessed using object recognition. The percentage of recognition during a 5 min exploration session was used as episodic memory index.

Working memory was evaluated using the Y-maze test. Percentage alternation is an index of working memory and was calculated for each mouse in a 10 min session.

Anxiety was assessed using three different tests: (i) the elevated plus maze for which we evaluated the time spent in open arms measured during a 10 min session, (ii) the open field test for which we evaluated the time spent in the central area ± SEM in the overall 15 min session, and (iii) the marble burying test for which the number buried was determined after a 30 min session.

All test results are means ± SEM. Statistical analysis was performed using Student t-test except for the EPM results of Cohorts 2 and 3 where assumptions of normality were not met and the object recognition results of Cohort 1 where the assumption of equality of variance was not met. For these data, a Mann-Whitney test was performed. NS, non-significant.