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. 2022 Jan 17;9:792257. doi: 10.3389/fcell.2021.792257

FIGURE 4.

FIGURE 4

Harmine mitigated sepsis-induced cardiac dysfunction and increased survival. (A) Diagram of the animal experiment. Harmine (50 mg/kg) or an equal volume of a 0.5% CMC-Na solution was administered to mice daily for seven consecutive days. At the end of Day 7 and 1 h after the gavage of harmine or vehicle, mice were intraperitoneally injected with LPS (10 mg/kg) or an equal amount of normal saline (NS). After 12 h, the mice underwent echocardiograms and were then sacrificed for further experiments. (B) Representative images of M-mode echocardiograms from mice. NS = normal saline. (C) Survival of mice after LPS (25 mg/kg) or vehicle injection. Mouse survival was recorded every 12 h up to 96 h (D,E) Plasma levels of LDH (n = 5) and cTnI (n = 7). (F) Heart rate, (G) ejection fraction (EF), (H) fractional shortening (FS), (I) left ventricular end-systolic diameter (LVEDs), and (J) left ventricular end-systolic volume (LVESV). [(F–J), n = 5 mice in the harmine group and n = 8 mice in the remaining groups]. Data are presented as the means ± SEM, & p < 0.05 compared with the control group; *p < 0.05 compared with the LPS group.