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. 2020 Feb;61(2):234–241. doi: 10.2967/jnumed.119.233064

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FIGURE 2. — In vitro assessment of hybrid tracers. Shown is staining of extracellular expression of PSMA on C2-2B₄ cells after incubation with nuclear stain (Hoechst; in blue) and hybrid tracer (Cy5; in red), with half-maximal inhibitory concentrations (IC₅₀, measured with ¹²⁵I-EuK–I-BA [((S)-1-carboxy-5-(4-(iodo-benzamido)pentyl)carbamoyl)-l-glutamic acid] as competitive radioligand). (A) EuK-Cy5-mas₃. (B) EuK-(SO₃)Cy5-mas₃. (C) EuK-Cy5(SO₃)-mas₃. (D) EuK-(Ar)Cy5-mas₃. (E) EuK-Cy5(Ar)-mas₃. (F) Blocking (checkered) of PSMA with EuK–I-BA. Binding affinities are represented as mean ± SD.