Table.
Specifics of Antibodies
| Antibodies | Source | Concentration, µg/mL | Catalog # | Lot # | RRID |
|---|---|---|---|---|---|
| Primary | |||||
| CGRP rabbit polyclonal whole serum | Immunostar (Hudson, WI, USA) | Cornea—1:4000 Skin—1:1000 | 24112 | 1714002 | AB_572217 |
| Tubulin β III mouse monoclonal IgG2a | Biolegend (San Diego, CA, USA) | 1 | 801202 | B205807 | AB_2313773 |
| PGP 9.5 mouse monoclonal | EnCor Biotechnology (Gainesville, FL, USA) | 1 | MCA-BH7 | 120718 | AB_2572394 |
| Secondary | |||||
| Alexa Fluor 488 goat anti-mouse, IgG2a | Jackson ImmunoResearch (West Grove, PA, USA) | 0.8 | 115-545-206 | 133801 | AB_2338855 |
| Alexa Fluor 546 goat anti-rabbit | Life Technologies (Carlsbad, CA, USA) | 1 | A11035 | 1904467 | AB_2534093 |
Concentration: 1.88 µg/ml; donkey anti-mouse Cy3 (715–165-151, lot 132845); donkey anti-goat Cy2 (705–225-174, lot 1333888). They were purchased from Jackson ImmunoResearch (West Grove, PA).