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. 2022 Jan 22;50:102248. doi: 10.1016/j.redox.2022.102248

Fig. 4.

Fig. 4

Decreased cell proliferation contributes to the impeded mesodermal differentiation in TFAM-/- cells

(A) Differentially expressed genes in PM stage treated with or without Dox.

(B) GSEA revealed the enrichment of the mesoderm development between hPSCs stage and PM stage treated with or without Dox.

(C) GO and KEGG analysis in the group treated with or without Dox.

(D) The heatmap displays the Log2FC in cell proliferation genes.

(E) Representative immunostaining images and quantification of T+ (red) and DAPI (blue) in PM stage treated with Dox/EdU or without Dox. Scale bars, 200 μm. Data were shown as Mean ± SEM (n = 3, *p < 0.05, n = 3, ***p < 0.001 compared with the +Dox group).

(F) Representative western blot TFAM in PM stage treated with Dox/EdU or without Dox. β-actin served as the loading control.

(G) Quantitative RT-PCR analysis of the expression level of TFAM, T in PM stage treated with Dox/EdU or without Dox. Data were shown as Mean ± SEM (n = 3, ***p < 0.001 compared with the +Dox group). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)