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. 2022 Jan 26;18:101351. doi: 10.1016/j.tranon.2022.101351

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© 2022 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 3 — HNF1A-AS1 suppressed miR-30b-5p expression by acting as a sponge. (A) Schematic diagram showing the predicted binding sites between HNF1A-AS1 and miR-30b-5p. (B) Dual luciferase reporter assay on HEK 293T cells with HNF1A-AS1-wt or HNF1A-AS1-mut was performed to confirm the interaction between HNF1A-AS1 and miR-30b-5p. (C) Cell lysate from MKN-45 cells incubated with anti-Ago2 antibody or IgG antibody for RIP. Expression levels of HNF1A-AS1 and miR-30b-5p were detected by qRT-PCR. (D) Effect of HNF1A-AS1 knockdown on miR-30b-5p expression level in MKN-45 cells analyzed by qRT- PCR. (E) Effect of HNF1A-AS1 overexpression on miR-30b-5p expression level in HGC-27 cells analyzed by qRT- PCR. (F) Correlation between HNF1A-AS1 and miR-30b-5p expression levels in GC tissues. Data were presented as mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001.