Fig. 9.

Representative wound closure photos of HCT116 cells after 72 h. (a) untreated HCT116 cells at 0 h, (b) untreated HCT116 cells at 72 h, (c) treated with IC50 of 5-FU (d) treated with IC50 of Diac (E) treated with IC50 of 5-FU+Diac. The wound width was measured in μm. Pictures were captured at 20× magnification and 1 mm scale bar. Panel (f) represents the average of the migration distance, where data are illustrated as means ± SD and analyzed using one-way ANOVA followed by Tukey’s Multiple Comparison test; P < 0.05. As compared with (*) HCT116-0hr, (#)HCT116-72 hr, (@) 5-FU and (ϕ) Diac-treated groups. ANOVA: analysis of variance; Diac: Diacerein; 5-FU: 5-fluorouracil; HCT116:human colorectal carcinoma cell line.