Figure 1.

Determination of selective disruption of Nos2 in adipose tissue. Mice containing flox sites before and after Exon 2 of Nos2 were created and backcrossed for at least 10 generations on a C57BL/6J background. Adipocyte-specific (Nos2^AD−KO) Nos2 knockout mice were generated on a C57BL/6J background by crossing mice homozygous for floxed Nos2 (Nos2^fl/fl) with Adipoq-Cre mice (A). Recombined alleles from genomic DNA isolation (B). ∗unspecific band. Δ: deletion. Nos2 expression in adipocytes isolated from epididymal (C), inguinal (D) and brown (E) adipose tissue of saline- and LPS-injected mice; N = 2–3.