Figure 2.

Nos2^AD-KOprevents HFHS diet increase in brown adipose tissue mass irrespectively of body weight gain.Nos2^AD−KO mice and wild-type (Nos2^fl/fl) mice were fed either a chow or a high-fat/high sucrose (HFHS) diet for 10 weeks. Total weight gain (A), total energy intake during 10 weeks (B), weight of brown adipose tissue (interscapular) (C). Nos2 mRNA expression in epididymal (D), inguinal (E) and brown (F) adipose tissue. Nitrite/nitrate content in epididymal (C), inguinal (D) adipose tissue and plasma (E). n = 11–12 mice/group. Two-way ANOVA with a Student-Newman-Keuls post hoc test was applied to calculate the significance of the differences between groups. Data are expressed as the mean ± SEM. ∗∗∗p < 0.001 for diet effect and ^#p < 0.05 for genotype difference.