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. 2022 Jan 14;57:101437. doi: 10.1016/j.molmet.2022.101437

Figure 3.

Figure 3

Adipocyte-specific knockout of Nos2 increases insulin sensitivity and alleviates diet-induced hyperinsulinaemia in HFHS-fed mice. 12 h fasting glycemia (A), insulinemia (B) and C-peptide (C). Mice 10 wk on HFHS diet were fasted overnight (12 h) and an oral glucose tolerance test (D) was performed after gavage with glucose (1 g/kg body weight). Blood samples were collected at each time point during OGTT for insulinaemia and C-peptide determination (E, F). Mice 8 wk on HFHS diet were 6 h fasted and an insulin tolerance test (E) was performed after an intraperitoneal injection of insulin (0.65 Ul/kg body weight). n = 11–12 (A, D, E); n = 8–11 (B); n = 7–11 (G), n = 3–6 (C, F). Two-way ANOVA with a Student-Newman-Keuls post hoc test was applied to calculate the significance of the differences between groups. Data are expressed as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 for diet effect; #p < 0.05 and ##p < 0.01 for genotype difference. HFHS, high fat/high sucrose; oGTT, oral glucose tolerance test; ipITT, intraperitoneal insulin tolerance test.