Fig. 4.

Intra-day inter-analyst precision (intra-day reproducibility) and inter-assay reproducibility from day 1 to day 4. One assay from each sample (R1 and R2 on day 1 and 2, buffy coat on day 3 and R3 on day 4) was conducted by two analysts. (A) The %CV of two assays from the same sample on the same day by different analysts was calculated as intra-day inter-analyst precision or intra-day reproducibility. Each data point represents %CV for an indicated subpopulation. The dotted line at 35% represents recommended precision for rare cell populations. (B) Inter-assay precision from all replicates from all assays. The dotted line at 10% represents the desired precision and at 35% represents recommended precision for rare cell populations.