Table 1.

Evaluation summary with cell recovery and viability. Ten assays were performed using three samples from RA patients (days 1, 2, and 4) and one buffy coat (day 3)

Assays performed	Sample code	HLA Type	No of Vials (Cells per vial at the time of freezing)	Frozen on	Cell count after thawing	Cell recovery after thawing (%)	Cell viability (%)
Day 1
Assay 1 Analyst 1	R1	DRB1∗04:01DRB1∗03:01	2 vials (17 × 106)	21/07/2016	3.56 × 106	10.47	72.00
	R2	DRB1∗01:01 DRB1∗16:01	2 vials (18 × 106)	15/06/2016	11.15 × 106	30.97	66.90
Assay 2 Analyst 2	R1	DRB1∗04:01DRB1∗03:01	1 vial (17 × 106)	21/07/2016	1.38 × 106	8.12	70.30
	R2	DRB1∗01:01 DRB1∗16:01	1 vial (18 × 106)	15/06/2016	3.93 × 106	21.83	84.10
Day 2
Assay 3 Analyst 1	R1	DRB1∗04:01DRB1∗03:01	3 vials (17 × 106)	21/07/2016	2.5 × 106	4.90	73.40
	R2	DRB1∗01:01 DRB1∗16:01	2 vials (18 × 106)	15/06/2016	6.48 × 106	18.00	87.10
Assay 4 Analyst 2	R1	DRB1∗04:01DRB1∗03:01	3 vials (17 × 106)	21/07/2016	3.88 × 106	7.61	88.00
	R2	DRB1∗01:01 DRB1∗16:01	1 vials (17 × 106)	15/06/2016	3.56 × 106	20.94	91.80
Day 3
Assay 5 Analyst 1	Buffy Coat	ND	36 × 106	16/10/2016	17.63 × 106	48.97	92.70
Assay 6 Analyst 2	Buffy Coat	ND	36 × 106	16/10/2016	17.26 × 106	43.53	92.30
Day 4
Assay 7 Analyst 1	R3	DRB1∗04:01 DRB1∗04:04	2 vials (18 × 106)	12/03/2020	21.93 × 106	60.92	93.40
Assay 8 Analyst 2	R3	DRB1∗04:01 DRB1∗04:04	2 vials (18 × 106)	12/03/2020	26.13 × 106	72.58	92.00
Replication
Assay 9 Analyst 1	R4	DRB1∗04:01 DRB1∗04:04	2 vials (20 × 106)	22/11/2019	25.7 × 106	68.75	98.40
Assay 10 Analyst 1	R5	DRB1∗04:01 DRB1∗04:01	2 vials (22 × 106)	15/06/2018	37.3 × 106	84.77	96.60

Cell count and viability determined with the Vi-Cell XR Cell Viability Analyzer.

ND, not determined.