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. 2021 Dec 5;207(1):84–94. doi: 10.1093/cei/uxab005

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© The Author(s) 2021. Published by Oxford University Press on behalf of the British Society for Immunology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — B cell activation with IL-17+BAFF+CpG led to colony formation, proliferation, and proportional increase in memory B cells. (A) Light microscopy images (top, representative images taken on day 5) and quantitative analysis per high power field (HPF) (bottom) of colony formation on days 5, 8, and 15 in ex vivo B cell cultures using different stimuli. Stimulation with IL-17+BAFF+CpG led to a larger number of colonies compared against all other conditions on day 5 (n = 2 independent experiments analysing 10 wells/condition) and compared to conditions lacking BAFF on day 8 and day 15. (B) IL-17+BAFF+CpG after 10 days led to a higher proportion of CD27^high in CD22⁺CD19⁺ B-cell cultures compared to other combination stimuli. (C) CFSE staining of live B cells (CD19⁺DAPI^-) show a higher fold increase in the proliferation index of IL-17+BAFF+CpG stimulated B cells compared to CpG (P = 0.04) (or IL-17+BAFF [P = 0.02]) on day 5 (n = 5 performed in two independent experiments).