Table 2.

IL-17+BAFF+CpG stimulation of B cells from patients with melanoma increases the frequency of identification of antigen-reactive B-cell clones

Patient	Stage	Day 0, Frequency (Freshly isolated and sorted cells out of total B cells)	Number of sorted cells (Day 0)	Day 7, Frequency (Freshly isolated and sorted cells out of total B cells)	Number of sorted cells (Day 7)
1	III	<1/31,000	3	<1/237	7
2	II	<1/9000	5	1/250	40
3	Ib	1/20,000	6	1/5000	7
4	IIa	<1/26,000	8	1/4200	2
5	IIb	1/30,000	25	1/25,000	20
6	IIIA	1/33,000	2	1/3,000	2

B-cell clones from patients with melanoma recognizing cancer cell-associated antigens conjugated on fluorescent beads were studied: a) from freshly isolated circulating B cells, selected by recognition of antigens conjugated on beads and sorted immediately; or b) after ex vivo culture with IL-17+BAFF+CpG for 7 days, prior to selection by binding to antigen-conjugated beads and single cell sorting. These suggest that these data suggest that individual B cell clones are likely to expand in response to innate stimuli and that the frequency of antigen-reactive clone selection was increased following ex vivo culture with IL-17+BAFF+CpG.