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Acetate, Butyrate, and Propionate inhibit MRC1 and Arg1 protein expression in AMs. MH-S were pretreated with Acetate, Butyrate, and Propionate 30 min at the indicated concentrations before IL-4 (20 ng/ml) exposure for 48 h. (A) Percentage of MRC1+ cells were determined by flow cytometry. (B) Immunofluorescence staining of the expression of Arg1. Data are shown as means ± SD from three independent experiments. ∗P < 0.05 vs. CON group. #P < 0.05 vs. IL-4 group.
