Fig. 5.

Butyrate and Propionate, but not Acetate ameliorate M2 polarization and allergic airway inflammation model. Mice were treated with Acetate, Butyrate, and Propionate in drinking water for 20 days before developing OVA-induced animal model. (A) Number of total cells and eosinophils in BALF. (B) IL-4, IL-5, and IL-13 levels in lung homogenates of mice. (C) Representative images of HE- and PAS-stained histologic sections of the lungs. Scale bars = 100μm. (D) Upper: gating strategy. Lower: Percentage of M2 population (MRC1+ cells) in the lung gated from viable CD45+F4/80+CD11c+ cells. (E) RT-qPCR was performed to evaluate mRNA levels of Arg1, MRC1, Fizz1, and Ym1 in the lung. Β-actin was used for normalization. Data are shown as means ± SD from three independent experiments. ∗P < 0.05.