Table 1.

Selected wildlife aquarium-based studies investigating the impact of biotic factors on PFAS levels in biological tissues. All findings listed under observations are reported as significant in the referenced publication.

Abiotic Factor	Species	n (treatment group; time point)	Total n	Species age attreatment	Duration of study	PFAS Investigated	PFAS Treatment Concentrations	Observations	Publication
Salinity	Marine Medaka (Oryzias melastigma)	6	300	3 months	2 wks & 4 wks	PFOS, PFOA, PFBS, PFDoDA	100 μ/L	Increase in salinity (0, 15, and 35 PSU) led to increased PFOS and PFOA in whole body concentrations Associated with increased gene and protein expression of FABP and OAT1 in the gill0	Avellán – Llagun o et al. 2020
	Blackrock fish (Sebastes schlegeli)	3	180*		60 days	PFOS, PFOA, PFDA, PFUnDA	10 μg/L	Reductions in salinity (34, 25, 17.5, 10 PSU) resulted in decrease d uptake and eliminatio n rate constants of PFDA, PFUnDA, PFOS but not PFOA in the serum. Serum and liver concentrations of individual PFCAs did not change over the 60-day period of investigation	Jeon et al. 2010 [90]
	Pacific oysters (Crassostrea gigas)	4	180*		56 days	PFOS, PFOA, PFDA, PFUnDA	10 μg/L	The distribution coefficients of PFOA, PFOS, PFDA, and PFUnDA in the whole oyster increased by 2.1- 2.7-fold as salinity increased from 10 to 34 PSU	Jeon al. 2010 [91]
Temperature	Rainbow trout (Oncorhy nchus mykiss)	5	200	15 months old	56 days	PFOS and PFHxS	500 μg/kg per fish per day	Increased temperatures (from 7 to 19 °C) led to increased PFOS and PFHxS in liver but decreased concentrations in muscle	Vidal et al. 2019

^*estimated total n based on study design for publications where total n was not explicitly stated.