Fig. 5.

METTL14 is a target of piRNA-14633 in Siha and Caski cells. a Diagram of the interaction between piRNA-14633 and the 3’UTR of METTL14. b Wild/mutant-type 3’UTR of METTL14 luciferase pmirGLO reporter vector. c, d Measurement of relative luciferase activity in Siha and Caski cells transfected with METTL14 -wild type or mutant-type, with 50 nM mi-14633 or 50 nM in-14633. e Real-time PCR was performed to detect the effect of piRNA-14633 (0 nM, 25 nM, 50 nM and 100 nM) on METTL14 mRNA expression f The correlation of piRNA-14633 with METTL14 expression in clinical FFPE specimens. *P < 0.05 vs. mi-NC group. ^#P < 0.05 vs. in-NC group. mi-14633 piRNA-14633 mimic, in-14633 piRNA-14633 inhibitor, NS not significant