Table 3. Combined Enzymatic Data.

compound	potently activea	moderately activeb	weakly activec
15		MYLK2 = 479 nM	DRAK2: 91%
8			MKNK2 = 2866 nM, DRAK2: 60%, YANK2 > 10000 nM
12		CSF1R = 233 nM
16	TYK2 = 33 nM	CSF1R = 305 nM	JAK2: 62%
21		TBK1 = 128 nM, TRKA = 477 nM	BMPR2 = 2918 nM, AURKB: 61%, JAK2: 107%
3	LRRK2 = 89 nM		DRAK2: 59%, NIM1 = 4398 nM, MYLK2 = 1119 nM
5		TBK1 = 149 nM	IKKε = 1199 nM
11		TBK1 = 187 nM, TRKA = 153 nM	JAK2: 87%, IKKε = 1314 nM, AURKB: 51%
17		DRAK1 = 325 nM, DRAK2 = 161 nM, AAK1 = 390 nM, SIK2 = 481 nM	MKNK2 = 769 nM, TYK2 = 720 nM, JAK2 > 10000 nM, BMP2K: 56%
19	CSF1R = 91 nM	TYK2 = 276 nM	JAK2: 54%, ERBB2 > 10000 nM, TBK1 = 2737 nM
GSK8612	TBK1 = 37 nM	LRRK2 = 159 nM, MAP2K5: 11%, CSF1R = 264 nM	IKKε = 552 nM, DAPK3 > 10000 nM, NUAK2 = 1151 nM, ULK3 = 946 nM, MKNK2 = 1369 nM
20	AURKB: 5%	NUAK1 = 176 nM, SIK2 = 185 nM	BMP2K: 58%, DRAK1: 78%, DRAK2: 65%, JAK2: 81%, ACVR1 = 537 nM, BMPR1B = 9028 nM
4	LRRK2 = 19 nM, DRAK2 = 62 nM	IKKε = 216 nM, DRAK1 = 202 nM, ULK3 = 343 nM, MKNK2 = 380 nM, TBK1 = 192 nM, MAP2K5: 12%	ULK2 = 1742 nM, ULK1: 58%
13	AURKB: 6%, BMP2K = 40 nM, AAK1 = 74 nM, TYK2 = 48 nM, STK16 = 82 nM	TBK1 = 192 nM, JAK2: 12%, BMPR2 = 488 nM, TRKA = 283 nM, NUAK1 = 317 nM	NUAK2 = 900 nM, CSNK2A2 = 2269 nM, PIP5K1A > 10000 nM, MKNK2 = 1009 nM
6	TBK1 = 66 nM, JAK2: 3%, BMP2K = 38 nM, STK16 = 88 nM, TYK2 = 67 nM, AURKB: 7%, AAK1 = 80 nM	CSF1R = 192 nM, TRKA = 158 nM, NUAK1 = 171 nM	PRP4 > 10000 nM, PIP5K1A > 10000 nM, CSNK2A2 = 2304 nM, CSNK2A1 > 10000 nM, ULK3 = 1371 nM

^aPotently active: IC₅₀ value < 100 nM or < 10% control at 1 μM (Table S1). All IC₅₀ values determined in duplicate.

^bModerately active: IC₅₀ value 200–500 nM or 10–49% control at 1 μM (Table S1). All IC₅₀ values determined in duplicate.

^cWeakly active: IC₅₀ value > 500 nM or > 49% control at 1 μM (Table S1). All IC₅₀ values determined in duplicate.