Figure 4.

Assessment of the accuracy of subunit counting under real experimental conditions and challenging stoichiometric configurations. Kernel density distribution functions and error measurements for 12-mer stoichiometries based on the addition of (a) monomeric or (b) dimeric units before and after refinement simulated at an SNR of 10.74 (photon count of 10) and intercomplex variation in photon count of 0%. Kernel density distributions are colored gray, and the Gaussian mixture is colored red. Error measurements based on the addition of (c–f) monomeric, (g–j) dimeric, or (k–n) trimeric units of high-order oligomers: (c, g, and k) 12-mer, (d, h, and l) 16-mer, (e, i, and m) 20-mer, and (f, j, and n) 24-mer. Simulations were performed at an SNR of 5.68 (photon count of 5) and an intercomplex variation in photon count of 20%. Proportions of each species can be found in the supplementary data.