. Author manuscript; available in PMC: 2022 Dec 6.

Published in final edited form as: Analyst. 2021 Dec 6;146(24):7464–7490. doi: 10.1039/d1an01560e

Table 3.

Significant correlations between Raman properties and mechanical properties of bone.

Mechanical test	Species bone	Raman property	Mechanical property	Correlation coefficient (r)	Ref
Three-point bending	Mouse femur	ν₁PO₄/Proline^a	PYD^d	−0.756	¹³⁴
Three-point bending	Mouse femur	ν₁PO₄/Proline^a	Ultimate stress	+0.810	¹³⁴
Nanoindentation	Mouse tibia	ν₁PO₄/Proline^a	Modulus	+0.565	¹³⁴
Nanoindentation	Mouse tibia	ν₁PO₄/Proline^a	Hardness	+0.567	¹³⁴
Three-point bending	Mouse femur	ν₁PO₄/Proline^a,b	Modulus	+0.796	¹³⁵
		ν₁PO₄/Proline^a,b	Stiffness	+0.666
		CO₃/ν₁PO₄^a,c	PY energy	+0.597
		CO₃/ν₁PO₄^a,c	PY toughness	+0.585
		1/FWHM[ν₁PO₄]^c	PY energy	+0.740
		1/FWHM[ν₁PO₄]^c	PY toughness	+0.770
Three-point bending	Rat femur	ν₁PO₄/AmideI^a	Stiffness	+0.663	⁵⁹
		ν₁PO₄/AmideI^a	Modulus	−0.793
		CO₃/ν₁PO₄^a	Stiffness	+0.824
		CO₃/ν₁PO₄^a	Modulus	−0.574
		1/FWHM[ν₁PO₄]	Stiffness	+0.678
		1/FWHM[ν₁PO₄]	Modulus	−0.592
Fatigue in tension	Human femur	1/FWHM[ν₁PO₄]	Modulus	+0.397	¹³⁶
			Yield stress	+0.266
			Fracture stress	+0.258
Three-point bending	Mouse femur	ν₁PO₄/AmideI^a	K_Ic^e	−0.836	¹³⁷
Three-point bending	Mouse femur	1/FWHM[ν₁PO₄]	K_Ic^e	−0.774	¹³⁷
Three-point bending	Human femur	ν₁PO₄/Amide I^a	K_Ic^e	+0.341	¹³⁸
Three-point bending	Human femur	CO₃/ν₁PO₄^a	ΔK_Ic/Δa^f	−0.278	¹³⁸
Tensile	Human femur	I₁₆₆₃/I₁₆₃₂^a	Ultimate strain	+0.740	¹³⁹
Tensile	Human femur	CO₃/ν₁PO₄^a	Ultimate strain	−0.880
Three-point bending	Human femur	I₁₆₆₃/I₁₆₃₂^a	Ultimate strain	+0.870
Three-point bending	Human femur	CO₃/ν₁PO₄^a	Ultimate strain	−0.750
Reference point indentation	Rat tibia	ν₁PO₄/Amide I^b	ID1	+0.770	¹²²
Reference point indentation	Rat tibia	CO₃/ν₁PO₄^b	ID1	−0.653	¹²²
Nanoindentation	Rat humerus	ν₁PO₄/Amide I^a	Modulus	+0.894	¹⁴⁰
Nanoindentation	Sheep femur	ν₁PO₄/CH₂ wag^a	Modulus	+0.387	¹⁴¹
Nanoindentation	Sheep femur	CO₃/ν₁PO₄^a	Modulus	+0.583	¹⁴¹
Nanoindentation	Mouse femur	ν₁PO₄/(hyd+pro)^b	Hardness	−0.883^g	¹²⁴
Nanoindentation	Mouse femur	ν₁PO₄/(hyd+pro)^b	Plastic index	+0.914^g	¹²⁴
Nanoindentation	Rat femur	ν₁PO₄/Amide I^a	Hardness	+0.787	¹²¹
Nanoindentation	Rat femur	CO₃/ν₁PO₄^a	Hardness	+0.632	¹²¹
Nanoindentation	Human vertebrae	ν₁PO₄/CH₂ wag^a	Modulus	+0.690	¹⁴²
Three-point bending	Bovine femur	I₁₆₇₀/I₁₆₄₀^a	Toughness	−0.616	⁶⁷
Three-point bending	Bovine femur	I₁₆₇₀/I₁₆₄₀^a	PY toughness	−0.735	⁶⁷
Three-point bending	Human femur	ν₁PO₄/AmideI^a	K_Ic^e	−0.396	⁵¹
Three-point bending	Human femur	I₁₆₇₀/I₁₆₄₀^a	K_Ic^e	−0.569	⁵¹
Nanoindentation	Human femur	1/FWHM[ν₁PO₄]	Modulus	+0.514	¹⁴³

Peak intensity ratio

Band area ratio

Correlation between osteogenesis imperfecta (OI) control and OI anti-TGF-β for Col1a2^+/p.G610C missense model are reported

Post-yield (PY) displacement (PYD)

Crack initiation toughness was determined as critical stress intensity factor K in mode I opening (K_Ic)

Crack growth toughness was determined as the change in per change in crack length 90 ΔK_Ic/Δa

Partial correlation