. Author manuscript; available in PMC: 2022 Dec 6.

Published in final edited form as: Analyst. 2021 Dec 6;146(24):7464–7490. doi: 10.1039/d1an01560e

Table 5.

Age-related difference in Raman spectroscopy properties of cortical bone as determined by baseline linear correction with and without single peak Gaussian fitting. The highest standard mean difference (d) was bolded for each property (filtered spectra).

Baseline	Method	6-mo.		20-mo.		Effect size (d)	p-value
Baseline	Method	Mean (SD)	COV (%)	Mean (SD)	COV (%)	Effect size (d)	p-value
Without correction	Direct	0.0512 (0.0005)	1.05	0.0521^a (0.0011)	2.09	1.065	0.0042
Without correction	Gaussian Fit	0.0496^a (0.0004)	0.867	0.0505^a (0.0008)	1.63	1.389	0.0003
Linear correction	Direct	0.0534 (0.0004)	0.825	0.0542 (0.0009)	1.80	1.077	0.0031
Linear correction	Gaussian Fit	0.0501 (0.0004)	0.851	0.0509^a (0.0008)	1.67	1.208	0.0014

Did not pass the Anderson-Darling normality test; therefore, the p-value was determined by the Mann-Whitney test. Otherwise, the comparison between 6-mo. and 20-mo. old mice was tested for significance using a two-sided t-test.