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. 2022 Jan 31;41:44. doi: 10.1186/s13046-022-02252-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — TRPM7 silencing reduces the expression of glycolysis-related regulators, but enhances the OXPHOS-related regulators in ovarian cancer cells. A IHC analysis of TRPM7, HK2, PDK1, IDH3B and UQCRC1 expression in human ovarian cancer tissues and non-tumor ovarian tissues. Scale bar = 50 μm. B Correlation analysis between the levels of TRPM7 and HK2, PDK1, IDH3B, or UQCRC1 expression in ovarian cancer tissues. C-D Western blot and RT-qPCR analyses of HK2, PDK1, IDH3B and UQCRC1 expression in the indicated cells. E Immunofluorescent analysis of HK2, PDK1, IDH3B and UQCRC1 expression in ovarian cancer cells after stained with mouse anti-HK2, rabbit anti-PDK1, mouse anti-IDH3B, rabbit anti-UQCRC1 and subsequent Alexa Fluor™488-goat anti-mouse IgG and Alexa Fluor™ 594-goat anti-rabbit IgG as well as DAPI, scale bar = 50 μm. F Western blot analysis of PKM2 in the nuclear and cytoplasmic fractions following TRPM7 silencing in SKOV3 and HO8910 cells. G IHC analysis of HK2, PDK1, IDH3B and UQCRC1 expression (scale bar = 50 μm)