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. 2022 Jan 30;10:21. doi: 10.1186/s40168-021-01221-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Metagenomic variation across feeding types, recovered from co-assembled samples. A Rarefaction curves of gene calls (ORFs) found because of sequencing depth. Curves are coloured according to feeding type, as indicated in legend. B Coefficient plot based on Tukey HSD of COG coverage between feeding types. Dots indicate Tukey’s HSD estimate and lines indicate confidence intervals. The shade of green indicates the relation between the estimate, confidence intervals, and null, meaning darkest green indicates significant estimates (p < 0.05). C Network of 17,354 COGs recovered from all samples. Big nodes indicate samples and small nodes indicate COGs. Distance between samples is based on similarity of samples. The size of sample nodes is based on the number of COGs connected to samples. The colour of COG nodes is based on the connectivity to sample nodes, where sample nodes are coloured as indicated in the legend. D Density plot and bar plot of bacterial SCGs are used to estimate the number of genomes in metagenomes. The dashed line in the bar plot indicates the highest density found of counted bacterial SCGs