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. 2022 Jan 30;10:21. doi: 10.1186/s40168-021-01221-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Genome-resolved metagenomics from gut microbiome across feeding type and intestinal sections. A Three metagenome-assembled genomes (MAGs) were resolved from the metagenome, consisting of 5.006 megabases (Mb) and 5574 non-redundant genes, which were visualised as yellow for Unknown Enterobacteriaceae, green for Candidatus Mycoplasma salmoninae mykiss, and red for Unknown Lactobacillus. Barplots indicate relative abundance of the two MAGs within each sample. Grouping of rainbow trout reared on different feeding types were visualised as orange for CTRL, blue for PRO, and red for SYN. Intestinal sections (gut sections) are coloured as black for mid gut and grey for distal gut. Heatmap visualises a series of genes of interest, which are related to isoprenoid biosynthesis, arginine biosynthesis, and polymer utilisation. Intensity of blue colour indicates log₁₀ of coverage of genes across samples. B Volcano plot of differentially abundant metagenomic genes between samples from CTRL vs. PRO and SYN. Colouration of nodes indicate significance, whereas red nodes are significant genes after correction and have a log₂ fold change (FC) > 1. Grey nodes are non-significantly differentiated genes between feeding types.