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. 2022 Jan 31;23:40. doi: 10.1186/s13059-022-02612-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — FIONA1 installs m⁶A modification on U6 snRNA and poly(A)⁺ RNA. a LC-MS/MS quantification of the m⁶A/A ratio in nuclear total RNA in 12-day-old Col-0 and fiona1-1 seedlings. b LC-MS/MS quantification of the m⁶A/A ratios of U6 snRNA in the indicated genotypic plant tissues. c-d Real-time fluorescence amplification curves and bar plot of the threshold cycle (C_T) of qPCR showing SELECT results for detecting m⁶A30 site in U6 snRNA with and without FTO demethylation treatment (c) and in Col-0 and fiona1-1 seedlings (d). Rn is the raw fluorescence for the associated well normalized to the fluorescence of the passive reference dye (ROX). e LC-MS/MS quantification of the m⁶A/A ratios of poly(A)⁺ RNA in the indicated genotypic plant tissues. Data are means ± SD for 3 biological replicates × 2 technical replicates. * p < 0.05, ** p < 0.01, *** p < 0.001 by t test (two-tailed). ns, no significance