Fig. 5.

Disruption of FIONA1 leads to transcriptome m⁶A hypomethylation. a Volcano plot of the hypomethylated m⁶A peaks identified in fiona1-1 across three biological replicates. b Cumulative fraction of log₂ (m⁶A enrichment fold change) for the hypomethylated m⁶A peaks in fiona1-1 and Col-0. Pie chart presenting RNA types of the confident hypomethylated m⁶A peaks identified in fiona1-1. d Metagene profile presenting the distributions of m⁶A peaks identified in Col-0 and fiona1-1 and the hypomethylated m⁶A peaks in fiona1-1 across the indicated mRNA segments. e Pie chart presenting the fractions of m⁶A peaks identified in Col-0 and fiona1-1 and the hypomethylated m⁶A peaks in fiona1-1 among non-overlapping transcript segments and the MEME-identified motifs for those m⁶A peaks. f Heatmap showing differentially expressed genes in two biological replicated Col-0 and fiona1-1 seedlings. g GO analysis of the hypomethylated m⁶A containing genes in fiona1-1. h GO analysis of the differentially expressed genes in fiona1-1 compared with Col-0