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. 2022 Jan 31;28:12. doi: 10.1186/s10020-021-00428-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Synergistic effects of ISL1 and KDM6B on SNAI1. A Expressions of KDM6B and SNAI1 in liver tissues of mice exposed to high-fat diet detected by Western blot analysis. B The correlation between ISL1 and SNAI1 in mouse liver tissues was analyzed by Pearson correlation analysis. C The correlation between KDM6B and SNAI1 in mouse liver tissues was analyzed by Pearson correlation analysis. D Expressions of KDM6B and SNAI1 in an in vitro model of lipid accumulation determined by Western blot analysis. E RT-qPCR was used to detect the expression of ISL1/KDM6B/SNAI1 in THLE-2 cells after overexpressing or knockdown of ISL1/KDM6B. F Western blot was used to detect the expression of ISL1/KDM6B/SNAI1 in THLE-2 cells after overexpressing/knockdown of ISL1/KDM6B. G Co-IP detection of the binding between ISL1 and KDM6B in vitro. H Immunofluorescence detection of the localization of ISL1 and KDM6B in cells. I Oil red O staining shows steatosis in response to artificial modulation of ISL1 and KDM6B expression. J ChIP detection of effect of overexpressing/knockdown of KDM6B on KDM6B enrichment and methylation degree in SNAI1 promoter region. *p < 0.05, ***p < 0.001 vs. NC treatment; ^###p < 0.001 vs. oe-ISL1 treatment. Cell experiment was repeated 3 times independently. n = 8 mice in each group