Fig. 5.

KDM6B and SNAI1 repressed lipogenesis and promoted lipolysis. A Western blot analysis was used to detect the KDM6B overexpression efficiency in liver tissues of mice exposed to high-fat diet. B Western blot analysis was used to detect the SNAI1 overexpression efficiency in liver tissues of mice exposed to high-fat diet. C Oil red O staining was applied to detect the effect of overexpressing KDM6B and SNAI1 on liver lipid accumulation in mouse model. D Morphology of liver tissues affected by overexpressing KDM6B and SNAI1 detected by HE staining. E Effect of overexpressing KDM6B and SNAI1 on plasma contents of TG and TC in mice exposed to high-fat diet. F Effect of overexpressing KDM6B and SNAI1 on the contents of TG and TC in liver tissues of mice exposed to high-fat diet. G Western blot analysis was conducted to detect the effect of KDM6B and SNAI1 on lipid synthesis- and lipolysis-related genes in liver tissues of mice exposed to high-fat diet. H Oil red O staining was performed to detect the effect of KDM6B and SNAI1 on lipid accumulation. I Western blot analysis was used to detect the effect of overexpressed KDM6B and SNAI1 on the expressions of KDM6B, SNAI1, lipid synthesis- and lipolysis-related genes in an in vitro model of lipid accumulation. ***p < 0.001 vs. oe-NC treatment. Cell experiment was repeated 3 times independently. n = 8 mice in each group