Fig. 2.

Flow cytometry gating strategy of maturation stages of T cell by using T CD4 and CD8 lyotubes kit. A representative flow cytometry plot from a vaccinated individual showing: Identification of CD4⁺ (a, b, c) and CD8⁺ (g, h, i) leukocytes non debris singlets. After gating on CD3⁺CD4⁺ T cells (d) and on CD3⁺CD8⁺ T cell (j) we identified CD45RA⁺CCR7⁺ naive cells; CD45RA⁻CCDR7⁺ central memory T cells (Tcm); CD45RA⁻CCR7⁺ effector memory T cells (Tem) and CD45RA⁺CCR7⁻ terminal differentiated effector memory T cells (Temra) (e). Same gating strategy is applied to T CD8⁺ cells (k). Regulatory T cells were classified as CD4⁺CD25⁺CD127^dim (f).