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. 2021 Sep;32(3):228–275. doi: 10.7171/jbt.21-3203-017

FIGURE 1.

FIGURE 1

“We have a LAMP for that”: major design choices when developing RT-LAMP tests. At each stage in the design process, a series of decisions affect the final configuration of the test, be that for an individual patient or for surveillance testing with pooled screening. The inherent flexibility and comparative simplicity of LAMP means that, for almost all settings and uses, there is 1 configuration of the LAMP toolbox that is fit for the purpose. The LAMP tests for use in any 2 settings or geographies can be dramatically different and can use NPS, ANS, OPS, TCEP, EDTA, DIY, HNB, LCV, DARQ, QuasR, OSD, or MolBeac. Abbreviations: ANS, anterior nares swab; DIY, do-it yourself; DARQ, dark quenching technique; EDTA, ethylenediamine tetraacetic acid; HNB, hydroxynaphthol blue; LAMP, loop-mediated isothermal amplification; LCV, leuco crystal violet; MolBeac, Molecular Beacons. NPS, nasopharyngeal swabs; OPS, oropharyngeal swab; OSD, oligonucleotide strand displacement; QuasR, quenching of unincorporated amplification signal reporters; RT-LAMP, reverse transcription–LAMP; TCEP, tris(2-carboxyethyl) phosphine.