FIGURE 7.

LAMP detection methods overview. A) Visual endpoint readouts use dyes that exhibit simple color or turbidimetric changes upon amplification. B) Similar to qPCR, real-time detection methods use fluorescent dyes to monitor the increase in viral load as the amplification progresses. The fluorescent signal can be sequence-independent (e.g., DNA intercalating; see “Sequence-Independent Detection of RT-LAMP Amplification Products”) or sequence-dependent (hybridization-based; see “Sequence-dependent detection of RT-LAMP amplification products”). C) The loop-mediated isothermal amplification (LAMP) products can, in principle, be verified by agarose gel electrophoresis followed by DNA staining, although that requires postamplification manipulation and the corresponding very real risk of between-experiment cross-contamination.