FIGURE 10.

Schematic of the diagnostics with coronavirus enzymatic reporting (DISCoVER) loop-mediated isothermal amplification (LAMP)-CRISPR-Cas (Agrawal et al. 2020). Viral RNA is reverse transcribed and amplified via LAMP then converted back to RNA using T7 polymerase. Cas13 enzymes are programmed with a guide RNA to specifically recognize the desired RNA molecules over non-specifically amplified products. Subsequent activation of Cas13 ribonuclease activity results in cleavage of quenched fluorescence reporter molecules. The CRISPR-Cas provides additional layers of specificity and sensitivity, albeit at increased cost and complexity.