TABLE 2.
(Extended)
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LAMP Reaction
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Further Information
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Amplification, min
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LoD (copies/reaction)
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Detection
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Enzyme Mix
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Volume/input, μL
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Method notes
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Source, y
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| 60 | 1–50 | Colorimetric (pH); fluorometric | NEB E1700; NEB M0380; NEB M0538; NEB M1800 | 10–25/5 | Used TTTT linker between F1c, F2 regions in FIP and BIP; saliva as matrix | Rabe and Cepko 2020 |
| 30 | Colorimetric (pH); fluorometric | NEB M0372; NEB M0380; NEB M0538 | 25/1 | LoD: 1 fg 8 mM MgSO+. 63°C. | Lamb et al. 2020 | |
| 20 | 10-1000 | Colorimetric (pH); fluorometric | NEB M1800 | 20/1–5 | Used GeneFinder nucleic acid stain (D039); Mineral oil to avoid evaporation | Yu et al. 2020 |
| 20-40 | 10–40 | Colorimetric (pH); fluorometric | NEB M1800 | 20/3 | Combiplex N2, E1 As1e primers. Guanidine chloride to enhance sensitivity | Zhang et al. 2020 |
| 60 | 5–250 | Fluorometric | NEB M0538; NEB M0380 | 16/2 | N gene via 2-step protocol to maximize sensitivity. RT then cDNA amplification. | Ganguli et al 2020 |
| RPA 20; LAMP 40 | 4 | Fluorometric; colorimetric (LCV) | OptiGene ISO-001 | 50/1 | Two-stage amplification. RPA 38°C; LAMP 63°C | El-Tholoth et al 2020 |
| ND | <100 | Colorimetric (pH); fluorometric | NEB M0538; NEB M1800 | 20/1 | Lalli et al 2021 | |
| 40–50 | Dependent on primers | Colorimetric (pH) | NEB M1804 | 20/0.5–1.9 | Superior performance with Orf7a and Orf3a primer sets. Singleplex and multiplex, compared with Sherlock | Schermer et al 2020 |
| 25 | 100 | Fluorometric | NEB E1700; NEB M0380 | 25/5 | Notable for above-average amplification temperature. 67°C | Mautner et al 2020 |
| 60 | 25 | Colorimetric (pH) | NEB M1800 | 25/5 | Nawattanapaiboon et al 2021 | |
| 20–30 | 10 | Fluorometric | NEB M0538; NEB M0380 | 10/2 | RT-LAMP coupled with CRISPR–Cas12-based lateral flow. | Broughton et al 2020 |
| 30 | 25 | Fluorometric | NEB M0538; NEB M0380 | 25/12 | LAMP primers are reconstituted freshly each day; 40 mM guanidine hydrochloride pH 8.0 | Mohon et al 2019 |
| 30 | 100 | Colorimetric (pH) | NEB M0538; NEB M0380 | 10 / 2 | Much greater primer concentrations than typical LAMP | Baek et al 2020 |
| 30 | 80 | Colorimetric (pH) | NEB M0538; NEB M0380 | 25/1 | Used FAM labeled FIP primer for fluorometric detection. | Huang et al 2018 |
| 30–40 | 118 | Colorimetric (pH); fluorometric | NEB M0374; NEB M0380; NEB M0491 | 25/3 | RT-LAMP on mismatch-tolerant amplification | Lu et al 2020 |
| 50 | 6 | Colorimetric (pH); fluorometric | NEB M0374 | 50/3 | Simplified water bath setup for visual RT-LAMP; 55–61°C | Wang et al 2020 |