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. 2021 Dec 10;13(3):827–841. doi: 10.1016/j.jcmgh.2021.12.003

Figure 7.

Figure 7

msRNA 23487 from E fergusonii–induced lipid accumulation by interacting with PPARα. (A) Western blot for PPARα protein in liver. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is shown as a loading control for liver protein (upper panel); binding sites of PPARα messenger RNA with msRNA 23487 predicted by miRanda software (lower panel). (B) Expression levels of msRNA 23487 and PPARa after transfection with msRNA 23487 NC or mimic in Huh7 and HepG2 cell lines. (C) PPARα protein levels after transfection with msRNA 23487 NC or mimic in Huh7 and HepG2 cell lines. (D) Oil Red O staining after transfection with msRNA 23487 NC or mimic in Huh7 and HepG2 cell lines treated with palmitic acid (PA) at a concentration of 0.3 mmol/L. (E) Intracellular triglyceride levels after transfection with msRNA 23487 NC or mimic in Huh7 and HepG2 cell lines. ∗P < .05, P > .01; ∗∗P < .01, P > .001.