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. 2022 Jan 12;42(2):166–182. doi: 10.1523/JNEUROSCI.2926-20.2021

Figure 3.

Figure 3.

Gephyrin regulates KCC2 membrane expression and clustering in Neuro2a cells. A, Representative confocal maximum projection images of Neuro2a cells cotransfected with plasmids encoding either GFP or GFP-tagged gephyrin (Gphn-GFP) and a plasmid encoding Flag-tagged KCC2. Cells were immunostained for surface and total KCC2 using anti-Flag antibodies. Right, Single confocal image showing overlay of surface Flag (red) and GFP (green) immunostainings. Note the colocalization of surface Flag-KCC2 and GFP-gephyrin immunofluorescent clusters (arrowheads). Scale bar, 5 µm. B, Summary boxplots showing normalized surface/total Flag immunofluorescence intensity ratios (GFP, n = 18; GFP-gephyrin, n = 20 cells; three independent experiments). **p < 0.01, Mann–Whitney rank-sum test.