Table 3.
qPCR primers
| Target | Refseq (NCBI) | Amplicon (bp) | Primer sequences |
|---|---|---|---|
| CNP1 | NM_001146318.1 | 147 | F: GAA GAA TAC GCC CAG CAG GA R: CAG ATC ACT GGG CCA CAA CT |
| Col1A1 | NM_007742.4 | 141 | F: GAC GCA TGG CCA AGA AGA CA R: CAT TGC ACG TCA TCG CAC AC |
| Col1A2 | NM_007743.3 | 139 | F: GGG TGT TCA AGG TGG CAA AG R: ACC AGG GAG ACC GAA TTC AC |
| Col3A1 | NM_009930.2 | 110 | F: TTG TGC AAA GTG GAA CCT GG R: ATA GGA CTG ACC AAG GTG GC |
| Dhh | NM_007857.5 | 141 | F: CAG ACC GCC TGA TGA CAG AG R: AGT GGA GTG AAT CCT GTG CG |
| Egr2 | NM_001347458.1 | 155 | F: GCC CCT TTG ACC AGA TGA AC R: GGA GAA TTT GCC CAT GTA AGT G |
| Gli1 | NM_010296.2 | 136 | F: GGA ATT CGT GTG CCA TTG GG R: GGA CTT CCG ACA GCC TTC AA |
| Gln | NM_177350.5 | 139 | F: AGC AGA AGA TCC AAA GGC CC R: CTG TTT GTG GTG AAT GGC GT |
| Hprt1 | NM_013556.2 | 131 | F: GTT AAG CAG TAC AGC CCC AAA R: AGG GCA TAT CCA ACA ACA AAC TT |
| Shh | NM_009170.3 | 135 | F: GGA AGA TCA CAA GAA ACT CCG AAC R: GAT GGC CAA GGC ATT TAA CTT GT |