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. Author manuscript; available in PMC: 2022 Nov 23.
Published in final edited form as: Adv Mater Interfaces. 2021 Oct 22;8(22):2101284. doi: 10.1002/admi.202101284

Figure 6. Phenotype of primary human hepatocytes (PHHs) in hydrogel-conjugated 96-well plates coated with select combinations of extracellular matrix (ECM) proteins.

Figure 6.

A) Select phase contrast images of PHHs on two stiffnesses (1 kPa and 25 kPa) coated with different combinations of ECM proteins. Scale bars = 50 µm. B) Cluster analysis for all measured functions of PHHs adhered to the selected combinations of ECM proteins on the two stiffnesses after 14 days of culture for a representative PHH donor. C) Scatterplot analysis of PHH donor-to-donor variability on different combinations of ECM proteins after 7 days of culture on 1 kPa (left) and tissue culture polystyrene (TCPS, no conjugated hydrogel) control wells (right) for CYP3A4 enzyme activities and D) albumin secretion.