Figure 6.

The recruitment of autophagy-related proteins limits invasion and protects cells from C. albicans-induced epithelial cell death.

(a) Representative images of C. albicans invasion assay performed in ATG16L1 WT or ATG16L1 KO HeLa cells at 4 h postinfection. Samples were processed for nuclei (blue), extracellular C. albicans (red), and total C. albicans (green) stainings. (b) Percentage of C. albicans invasion in ATG16L1 WT (black bar) or ATG16L1 KO (white bar) HeLa cells. Data are mean ± SEM of eight independent experiments. (c, d, and e) Percentage of cell death as measured by propidium iodide incorporation (PI+ cells) in ATG16L1 WT (black bar) or ATG16L1 KO (white bar) HeLa cells infected by C. albicans for (c) 4 h, (d) 6 h, or (e) 20 h postinfection. Data are mean ± SEM of six independent experiments. (f, g, and h) Percentage of cell death as measured by propidium iodide incorporation (PI+ cells) in Atg5 WT MEFs (black bar) or Atg5 KO MEFs (white bar) infected by C. albicans for (f) 4 h, (g) 6 h, or (h) 20 h postinfection. Data are mean ± SEM of seven independent experiments.