Skip to main content
. 2022 Jan 30;11(1):120–132. doi: 10.1080/21623945.2022.2030570

Figure 5.

Figure 5.

miR-669a-5p induces a brown adipocyte phenotype in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were transfected with mimic control or mimic miR-669a-5p (100 nm) on day 0 and day 4 after differentiation, cells were harvested on day 8 for analysis.(a) RT-qPCR to analyse the expression of thermogenic genes Pgc-1α and Ucp1 in 3T3-L1 adipocytes, normalized to β-actin expression. n = 3 per group.(b) Western blot to evaluate the protein levels of thermogenic genes PGC-1α and UCP1 in 3T3-L1 adipocytes, β-ACTIN was used as a loading control. n = 3 per group.(c) Quantitative densitometry of the Western blots showed in B.Data are representative of at least three individual experiments. Results are represented as mean ± SEM. *p < 0.05 versus mimic control group.