Figure 1.

FGF21 decreases body fat mass by increasing energy expenditure. Body weight (A) and fat mass (B) were measured throughout the study (n = 15–16). In week 8, food intake (C), physical activity (D), energy expenditure (E), fat oxidation rate (F), and carbohydrate oxidation rate (G) were monitored, and values shown (E–G) were corrected for body lean mass (n = 8). (A–D) Data are represented as mean ± SEM. (E–G) For line graphs, data are shown as mean for each group (12-h cycle; shaded area represents the dark cycle); for bar graphs, data are shown as mean ± SEM. Differences were assessed using unpaired two-tailed Student’s t-test. **P < 0.01, ***P < 0.001.