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. 2021 Feb 8;118(2):573–584. doi: 10.1093/cvr/cvab039

Figure 1.

Figure 1

EAM was induced in wild-type (WT) and in Agtr1a-/- mice by αMyHC/CFA immunization at day 0 and 7. Representative histology (H/E) and immunohistochemistry for CD45, CD3, and F4/80 in hearts of indicated recipients at day 21 (inflammatory phase) are presented in (A). Scale bar = 100 μm. Myocarditis severity scores and quantification of heart-infiltrating CD3+, CD45+, and F4/80+ cells in WT (n = 5) and Agtr1a-/- (n = 5) mice are shown in (B). t-SNE plot presenting cardiac CD45+-gated cell subsets identified by flow cytometry (gating strategy shown in FigureS 2) and quantification of the indicated subsets in hearts of WT (n = 6) and Agtr1a-/- (n = 6) mice at day 21 of EAM are shown in (C). Myocarditis severity scores of vehicle- (control, n = 6) or telmisartan-treated (n = 7) Agtr1a-/- mice at day 21 are presented in (D). Expression of selected profibrotic genes in cardiac tissue at day 21 is shown in (E). p values calculated with Mann–Whitney U test or unpaired Student’s t-test.