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. 2021 Feb 8;118(2):573–584. doi: 10.1093/cvr/cvab039

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© The Author(s) 2021. Published by Oxford University Press on behalf of the European Society of Cardiology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Inflammatory cells were isolated from hearts of wild-type (WT) and Agtr1a^-/- mice at day 17–21 of EAM, expanded, and stimulated with TGF-β1 (10 ng/mL) for 30 min or 24 h. Panel (A) shows anti-RhoA immunoblots of purified GTP-bound Rho protein extracts (RhoA-GTP) and total cell lysates (total RhoA). Panel (B) shows anti-RhoA and anti-AKAP-Lbc immunoblots (IB) of immunoprecipitated (IP) protein extracts with anti-AKAP-Lbc antibodies. Anti-RhoA immunoblots of the whole cell extracts are presented in the bottom panel. Panel (C) shows quantifications of normalized RhoA-GTP levels (normalized to total RhoA, left, n = 4) and amount of RhoA immunoprecipitated with anti-AKAP-Lbc antibodies (normalized to IB AKAP-Lbc, right, n = 3). p values calculated with unpaired Student’s t-test.