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. 2022 Jan 31;17(1):e0262231. doi: 10.1371/journal.pone.0262231

Fig 3. Numbers of IL-4- and IFN-γ-secreting splenocytes.

Fig 3

ELISPOT assays were used to detect IL-4- and IFN-γ-secreting lymphocyte cells in the spleen. Splenocytes were stimulated with F protein (expressed in 293F cells, 10 μg/mL) for 16 h. (A) Numbers of IFN-γ secreting splenocytes. (B) Numbers of IL-4 secreting splenocytes. (C) Ratio of IL-4-secreting splenocytes and IFN-γ-secreting splenocytes. Statistically significant differences were measured by one-way ANOVA with Newman–Keuls posttest. *** p<0.001, **p<0.01, * p<0.05.