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. 2022 Feb 1;132(3):e147191. doi: 10.1172/JCI147191

Figure 8. Neutrophilia by adoptive neutrophil transfusion accelerates AngII-induced cardiac hypertrophy.

Figure 8

(A) LV function and hypertrophy. Non-NT, no neutrophil transfusion; NT, neutrophil transfusion; NT-DN, neutrophil transfusion plus DNase I treatment. All groups received a 4-week AngII infusion (n = 5–9). (B) Plasma cardiac troponin I (cTnT) levels after 1-week AngII infusion (n = 5–8). (C) Myocardial hypertrophy (WGA staining) and fibrosis (Picrosirius red staining). (D) Intracardiac NETs (H3Cit) and cell death (TUNEL). (E) Histone-associated DNA fragments and cell-free DNA (cfDNA) in the plasma of HF patients and non-HF controls (n = 8). P values are from 1-way ANOVA with Tukey’s correction (A–D) or 2-tailed, unpaired Student’s t test (E). Representative images from an individual animal (n = 5–9 in each group). Scale bars: 25 μm.