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. 2022 Feb 1;132(3):e141573. doi: 10.1172/JCI141573

Figure 6. Hyperinflammation and collagen deficiency, but unchanged ROS levels, in ISG15–/– HaCaT keratinocytes.

Figure 6

HaCaT cells were stimulated with IFN-α (1000 IU/mL) for 12 or 24 hours and analyzed by RT-qPCR, enzyme-linked immunoassay, and flow cytometry. (A–E) Increased IFIT2 mRNA in cell pellets and IP10, TNF-α, IL-1β, and IL-6 in culture supernatants from ISG15–/– HaCaT cells. Increased concentrations of TNF-α and IL-1β were seen in ISG15–/– cells only after IFN-α stimulation. (F and G) Near absence of MMP1 mRNA but unchanged expression of TIMP1 mRNA in ISG15–/– HaCaT cells. (H–L) Decreased expression of several collagen mRNAs except COL15A1 in ISG15–/– cells. (M and N) Cellular and mitochondrial ROS. n = 3, ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001 (A–L, Student’s t test; M and N, 1-way ANOVA with Tukey’s post hoc test).