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. 2022 Feb 1;132(3):e151239. doi: 10.1172/JCI151239

Figure 6. The proportion of tissue resident–like CD8+ T cells with effector memory phenotype was significantly higher in the vaccinated tumor microenvironment.

Figure 6

Single-cell suspensions dissociated from tumor samples from arm 1 (4 cases) and arm 2 (6 cases) were analyzed by mass cytometry. (A) CD3+ T cells were subjected to dimension reductional algorithm t-SNE and clustered by FlowSOM based on the expression status of 10 differentiation markers (CD4, CD8a, CD62L, CD27, CD127, CCR7, CD45RO, CD45RA, CD25, and PD-1). (B) Heatmap visualizing the relative expression (z score) of T cell–relevant markers in each subpopulation. Each cluster was annotated based on the expression status of differentiation markers as listed above. (C) The proportion of tissue resident–like CD8+ T cells with effector memory phenotype (CD103+, PD-1+, CXCR3hi, CCR7–, CD45RO+, GZMBhi) was significantly higher in arm 1 samples. *P < 0.05 (nonpaired Wilcoxon test). The proportion of Tregs in arm 1 showed a trend toward a higher percentage than arm 2 but without statistical significance. (D) TILs in this tissue resident–like CD8+ T cell cluster in arm 1 tumors demonstrated significantly higher expression levels for the CXCL10 receptor CXCR3, GZMB, and Tbet than those in arm 2 tumors.