Figure 5.

AF confers therapeutic effects of antenatal MEx treatment to improve fetal lung branching morphology in preeclamptic pregnancies. (A) Experimental design. (B) Representative images of lung explants. Scale bars, 150 µm and 60 µm. (C) Quantification of average new branches per squared millimeter at the end of a 48-hour culture period. Data are representative of four independent experiments, with lung explants harvested from 3–4 pregnant dams per experiment plated into 4–5 explants per condition in each experiment. **P < 0.01. Control amniotic fluid (AF) = explants cultured with AF from control pregnancies; Media = explants cultured with media only; MEx = explants cultured with MEx alone (no AF); PE AF = explants cultured with PE AF; PE + MEx AF = explants cultured with AF from preeclamptic pregnancies treated with MEx therapy.