Figure 4.

DHCT reversed nicotine induces cell migratory, invasive, and EMT properties in Huh7 and HepG2 HCC cells. (a, b) Wound healing and Transwell assay, respectively, measured cell migration and invasion, after the DHCT treatment on nicotine (0.5 μM) stimulated HCC (Huh7 and HepG2) cells, bar plot adjacent indicates the percentage speed of wound healing and invasion between the DHCT + nicotine and nicotine treatment group. Scale bar 50 μm. (c) Western blot analysis of protein expression on HCC cells treated with DHCT efficiently reduced the expression of EMT process; induced E-cadherin, and reduced vimentin expression observed. GAPDH served as the loading control. Data from one of a representative experiment, (from at least three independent assays); ^∗p < 0.05 versus controls (Student's t-test).