Skip to main content
. 2021 Dec 17;21(2):100185. doi: 10.1016/j.mcpro.2021.100185

Fig. 4.

Fig. 4

Expression and functions of GFPT2 in the D492 EMT model.A, GFPT2 showed the highest expression in D492HER2 while lowest in D492 on the RNA level; B, The protein expression of GFPT2 in the three cell lines suggested highest expression of GFPT2 in D492HER2 confirmed by both LFQ (left) and SILAC (right); C, The GFPT2 level in D492DEE which was the negative control cell line of D492HER2 indicated that the increased expression of GFPT2 was not due to the artifacts from cell handling but the overexpression of HER2. D–F, siRNA-mediated knockdown of GFPT2 decreased VIM in both mesenchymal cell lines. G–I, knockdown of GFPT2 affected CDH2-to-CDH1 ratios in all cell lines. J–L, knockdown of GFPT2 decreased the growth of D492M (K) and D492HER2 (L) after 90 h from cell seeding. M and N, D492HER2 cell invasion was decreased after knockdown of GFPT2 and confirmed by two siRNAs. O, D492 cell migration was slowed by the knockdown of GFPT2. A decreasing trend was seen in D492M and D492HER2 without significance. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.